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Reactivity | Human |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Calculated MW | 175kDa |
Observed MW | Refer to figures |
Immunogen | A synthetic peptide of human EIF4G1 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | j |
Synonym | P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1; |
Western blot analysis of extracts of various cell lines, using EIF4G1 antibody.
Immunohistochemistry of paraffin-embedded Human liver injury using EIF4G1 antibody at dilution of 1:100 (x400 lens).
Immunofluorescence analysis of A549 cell using EIF4G1 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A549 cell using EIF4G1 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A549 cell using EIF4G1 antibody. Blue: DAPI for nuclear staining.
The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.
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