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Reactivity | Human Mouse Rat |
Tested applications | WB IHC |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
Calculated MW | 63kDa |
Observed MW | Refer to figures |
Immunogen | Recombinant protein of human EYA3 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Western blot analysis of extracts of various cells, using EYA3 antibody.
Immunohistochemistry of paraffin-embedded rat kidney using EYA3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using EYA3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric using EYA3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using EYA3 antibody at dilution of 1:100 (40x lens).
This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants.
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