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Reactivity | Human Mouse Rat |
Tested applications | WB IHC |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
Calculated MW | 211kDa |
Observed MW | Refer to figures |
Immunogen | Recombinant protein of human MYLK |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083; |
Western blot analysis of extracts of Mouse uterus, using MYLK antibody.
Immunohistochemistry of paraffin-embedded human gastric using MYLK antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded rat kidney using MYLK antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse kidney using MYLK antibody at dilution of 1:100 (x40 lens).
This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.
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