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sales@neoscientific.com
+1-888.733.6849
+1-617.299.7367 (Int’l)
+1-888.733.6849
+1-617.299.7367 (Int’l)
| Reactivity | Human Mouse Rat Other (Wide Range) |
| Tested applications | WB IHC IF IP CHIP CHIPseq |
| Recommended Dilution | WB 1:1000 - 1:3000 IHC 1:200 - 1:500 IF 1:500 - 1:1000 IP 1:200 - 1:500 CHIP 1:20 - 1:50 CHIPseq 1:20 - 1:50 |
| Calculated MW | 11kDa |
| Observed MW | Refer to figures |
| Immunogen | A synthetic peptide of human Acetyl-Histone H4-K8 |
| Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Concentration | u |
| Synonym | H4K8ac; H4; H4/n; H4F2; H4FN; FO108; HIST2H4; H4K8ac |
Western blot analysis of extracts of various cells, using Acetyl-Histone H4-K8 antibody.
Immunofluorescence analysis of HeLa cell using H4K8ac antibody. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis extracts of 293 cell line, using H4K8ac antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
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