CDK1-Monoclonal Antibodies

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CDK1

Qty


Total
$300
Catalog #
A0030
Antibody Type
Monoclonal Antibody
Gene ID
983
Swiss Prot
P06493
Size
Species
Mouse
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Rat
Tested applicationsWB ICC
Recommended DilutionWB 1:100 - 1:200 ICC 1:50 - 1:100
Calculated MW34kDa
Observed MWRefer to Figures
ImmunogenRecombinant protein of human CDK1
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentrationbd
SynonymCDC2;CDC28A;CDK1;DKFZp686L20222;MGC111195
Images
  • A0030: image 1

    Western blot analysis of extracts of various cells, using CDK1 antibody.

Background

The entry of eukaryotic cells into mitosis is regulated by CDK1 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of CDK1 at Thr161 (1). However, the critical regulatory step in activating CDK1 during progression into mitosis appears to be dephosphorylation of CDK1 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of CDK1, can be carried out by Wee1 and Myt1 protein kinases (3,4). The CDK15 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of CDK1 (1,5).

Protocol

N/A

MSDS
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