|Tested applications||WB ICC|
|Recommended Dilution||WB 1:100 - 1:200
ICC 1:50 - 1:100|
|Observed MW||Refer to Figures|
|Immunogen||Recombinant protein of human CDK1|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
The entry of eukaryotic cells into mitosis is regulated by CDK1 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of CDK1 at Thr161 (1). However, the critical regulatory step in activating CDK1 during progression into mitosis appears to be dephosphorylation of CDK1 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of CDK1, can be carried out by Wee1 and Myt1 protein kinases (3,4). The CDK15 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of CDK1 (1,5).
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