DDDDK-Tag-Monoclonal Antibodies

our services
our products
Quotations & Ordering:

For quotations, please use our online quotation form, and you may also contact us by

[email protected]

Phone:

+1-888.733.6849
+1-617.299.7367 (Int’l)
+44-203.695.3777 (UK)

Fax:

+1-888.733.6849
+1-617.299.7367 (Int’l)

Our customer service representatives are available 24 hours, Monday through Friday to assist you.

DDDDK-Tag

Qty


Total
$165
Catalog #
AE005
Antibody Type
Monoclonal Antibody
Gene ID
Swiss Prot
Size
Species
Mouse
Isotype
IgG
Purity
Affinity purification
Additional Information
Tested applicationsWB IHC IF IP
Recommended DilutionWB 1:2000 - 1:5000 IHC 1:50 - 1:500 IF 1:50 - 1:500 IP 1:50 - 1:100
Observed MWRefer to Figures
ImmunogenA synthetic Peptide of human DDDDK-Tag
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Images
  • AE005: image 1

    Western blot analysis of over-expressed DDDDK-tagged protein in 293T cell using DDDDK antibody at different dilution. Each lane was loaded with 2 ug cell lysate.

  • AE005: image 2

    Immunoprocipitation of over-expressed DDDDK-tagged protein in HeLa cell incubated with DDDDK antibody. A mock served as negative control and over-expressed Hela cell lysate served as positive control.

Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a mouse monoclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins.

Protocol

N/A

MSDS
Reviews
Neo Scientific welcomes feedback from its customers.

If you have used an our product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information.

If you have any additional inquiries please email technical services at [email protected].

Thank you for your support.

promotions

Image

Custom Monoclonal Antibody
MassAb Technologies
Image
Free Trial Custom Polyclonal Antibody
You will receive free purified antibodies for validation
Image
Custom Peptides Synthesis

Start from $40/each

new technologies

Image
Optimized for enhanced
expression levels
XPromoter 2.0
Image
E.coli/insect cells/293 & CHO

3 in 1 expression system
Image
MassAb Technologies

Cover All Epitopes