GAPDH -Monoclonal Antibodies

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GAPDH

Qty


Total
$135
Catalog #
AC002
Antibody Type
Monoclonal Antibody
Gene ID
2597
Swiss Prot
P04406
Size
Species
Mouse
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Mouse Rat
Tested applicationsWB IHC IF
Recommended DilutionWB 1:1000 - 1:5000 IHC 1:200 - 1:500 IF 1:100 - 1:200
Calculated MW36kDa
Observed MWRefer to Figures
ImmunogenRecombinant protein of human GAPDH
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
SynonymGAPDH;G3PD;GAPD;MGC88685 ;
Images
  • AC002: image 1

    Western blot analysis of extracts of various cell lines, using GAPDH antibody at 1:2000 dilution.

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocated to the nucleus post modification such as S-nitrosylation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Protocol

N/A

MSDS
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