GFP-Tag-Monoclonal Antibodies

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GFP-Tag

Qty


Total
$135
Catalog #
AE011
Antibody Type
Polyclonal Antibody
Gene ID
Swiss Prot
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
Tested applicationsWB IF
Recommended DilutionWB 1:2000 - 1:3000 IF 1:50 - 1:100
Observed MWRefer to Figures
ImmunogenRecombinant protein of GFP
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Images
  • AE011: image 1

    Western blot analysis of over-expressed GFP fusion protein in 293 cell using GFP-Tag antibody at different dilution. Each lane was loaded with 2 ug cell lysate.

  • AE011: image 2

    Immunoprocipitation of over-expressed GFP-Tagged protein in HEK-293 cell incubated with GFP-Tag antibody.

Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP.

Protocol

N/A

MSDS
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