MCM3-Monoclonal Antibodies

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MCM3

Qty


Total
$220
Catalog #
A1060
Antibody Type
Polyclonal Antibody
Gene ID
4172
Swiss Prot
P25205
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Mouse Rat
Tested applicationsWB IHC IF
Recommended DilutionWB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Calculated MW91kDa
Observed MWRefer to Figures
ImmunogenRecombinant protein of human MCM3
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentrationd
SynonymMCM3;HCC5;MGC1157;P1-MCM3;P1.h;RLFB ;
Images
  • A1060: image 1

    Western blot analysis of extracts of various cell lines, using MCM3 antibody.

  • A1060: image 2

    Immunohistochemistry of paraffin-embedded human oophoroma using MCM3 antibody at dilution of 1:100 (400x lens).

  • A1060: image 3

    Immunofluorescence analysis of HeLa cell using MCM3 antibody. Blue: DAPI for nuclear staining.

  • A1060: image 4

    Immunofluorescence analysis of U2OS cell using MCM3 antibody. Blue: DAPI for nuclear staining.

Background

The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is also a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,9).

Protocol

N/A

MSDS
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