|Reactivity||Human Mouse Rat Other (Wide Range)|
|Tested applications||WB IHC IF IP CHIP CHIPseq|
|Recommended Dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
CHIPseq 1:20 - 1:100|
|Observed MW||Refer to Figures|
|Immunogen||A synthetic methylated peptide corresponding to residues surrounding R8 of human histone H3|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonym||H3R8me1; HIST1H3J; H3/j ;H3FJ ; Histone H3.1; Histone H3/a; Histone H3/b;Histone H3/c; Histone H3/d; Histone H3/f;Histone H3/h ; Histone H3/I;Histone H3/j ; Histone H3/k; Histone H3/l; HIST3H3;|
Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3R8me1 antibody.
Dot-blot analysis of all sorts of methylation peptides using H3R8me1 antibody.
Immunofluorescence analysis of 293T cell using H3R8me1 antibody. Blue: DAPI for nuclear staining.
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
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