Phospho-EIF4E-S209-Polyclonal Antibodies

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Phospho-EIF4E-S209

Qty


Total
$300
Catalog #
AP0228
Antibody Type
Polyclonal Antibody
Gene ID
1977
Swiss Prot
P06730
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman
Tested applicationsWB IHC IF
Recommended DilutionWB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:100 - 1:200
Calculated MW25kDa
Observed MWRefer to Figures
ImmunogenA phospho specific peptide corresponding to residues surrounding S209 of human EIF4E
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
SynonymCBP; EIF4F; AUTS19; EIF4E1; EIF4EL1;
Images
  • AP0228: image 1

    Western blot analysis of extracts from SK-BR-3 cells, using Phospho-EIF4E-S209 antibody.

  • AP0228: image 2

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-EIF4E-S209 antibody.

  • AP0228: image 3

    Immunofluorescence staining of methanol-fixed MCF cells using Phospho-EIF4E-S209 antibody.

Background

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene.

Protocol

N/A

MSDS
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