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| Reactivity | Human Mouse Rat |
| Tested applications | WB IHC IF |
| Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:100 - 1:200 |
| Calculated MW | 60kDa |
| Observed MW | Refer to Figures |
| Immunogen | A phospho specific peptide corresponding to residues surrounding S394 of human HDAC2 |
| Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Concentration | fip |
| Synonym | HD2; RPD3; YAF1; |
Western blot analysis of extracts from 293 cells, using Phospho-HDAC2-S394 antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-HDAC2-S394 antibody.
Immunofluorescence staining of methanol-fixed Hela cells showing nuclear staining using Phospho-HDAC2-S394 antibody.
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.
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