Phospho-HSPB1-S82-Monoclonal Antibodies

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Phospho-HSPB1-S82

Qty


Total
$300
Catalog #
AP0041
Antibody Type
Polyclonal Antibody
Gene ID
3315
Swiss Prot
P04792
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Rat
Tested applicationsWB IHC IF
Recommended DilutionWB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Observed MWRefer to Figures
ImmunogenA phospho specific peptide corresponding to residues surrounding S82 of human HSPB1
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentrationj
SynonymCMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; DKFZp586P1322
Images
  • AP0041: image 1

    Western blot analysis of extracts of HeLa cell line, using Phospho-HSPB1-S82 antibody.

  • AP0041: image 2

    Immunofluorescence analysis of U2OS cell using Phospho-HSPB1-S82 antibody. Blue: DAPI for nuclear staining.

Background

Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).

Protocol

N/A

MSDS
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