|Reactivity||Human Mouse Rat|
|Tested applications||WB IHC IF IP RIP|
|Recommended Dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
RIP 1:20 - 1:50|
|Observed MW||Refer to Figures|
|Immunogen||Recombinant protein of human RAN|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
Western blot analysis of extracts of various cell lines, using RAN antibody.
Immunofluorescence analysis of HeLa cell using RAN antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of MCF-7 cell using RAN antibody. Blue: DAPI for nuclear staining.
Ran is a small GTPase of the Ras family that plays a central role in the spacial and temperal organization of eukaryotic cells. During interphase, Ran-GDP localizes to the cytoplasm and Ran-GTP to the nucleus. This polarized localization of Ran ensures its role in nuclear transport (1). During mitosis, Ran-GTP is chromatin associated, where it promotes spindle assembly and nuclear envolope formation (1,2). In S phase, Ran-GTP associates with and inhibits MCM helicase, ensuring precise chromosomal DNA duplication during the cell cycle (3)
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