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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF IP ChIP |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 |
Calculated MW | 47kDa |
Observed MW | Refer to figures |
Immunogen | Recombinant protein of human TEAD1 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | AA; REF1; TCF13; TEF-1; NTEF-1; TCF-13; TEAD-1; |
Western blot analysis of extracts of various cell lines, using TEAD1 antibody.
Immunohistochemistry of paraffin-embedded human lung cancer using TEAD1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human liver cancer using TEAD1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TEAD1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using TEAD1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using TEAD1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse lung using TEAD1 antibody at dilution of 1:100 (x40 lens).
Chromatin immunoprecipitation analysis extracts of HeLa cell line, using TEAD1 rabbit polyclonal antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified.
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