TGFB1-Monoclonal Antibodies

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TGFB1

Qty


Total
$300
Catalog #
A0291
Antibody Type
Monoclonal Antibody
Gene ID
7040
Swiss Prot
P01137
Size
Species
Mouse
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman
Tested applicationsWB IHC FC
Recommended DilutionWB 1:500 - 1:2000 IHC 1:50 - 1:200 FC 1:50 - 1:200
Calculated MW50kDa
Observed MWRefer to Figures
ImmunogenRecombinant protein of human TGFB1
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentrationbrt
SynonymTGFB1;CED;DPD1;TGFB;TGFbeta ;TGF-
Images
  • A0291: image 1

    Western blot analysis of extracts of A549 cell and HepG2 cell tissue, using TGFB1 antibody.

Background

Transforming growth factor-β (TGFB1) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-4). TGFB1 elicits signaling through three cell surface receptors: type I (RI), type II (RII) and type III (RIII). Type I and type II receptors are serine/threonine kinases that form a heteromeric complex. In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and activation of type I receptor kinases (5). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGFB1 with high affinity but lacks a cytoplasmic signaling domain (6,7). Expression of the type III receptor can regulate TGFB1 signaling through presentation of the ligand to the signaling complex. The only known direct TGFB1 signaling effectors are the Smad family proteins, which transduce signals from the cell surface directly to the nucleus to regulate target gene transcription (8,9).There are three isoforms of TGF-beta designated TGF-beta1, TGF-beta2, and TGF-beta3, which are encoded by distinct genes and are expressed in a tissue specific manner (10). Each of the isoforms are synthesized as larger precursor proteins containing a propeptide region which gets cleaved prior to secretion from the cell. Mature TGF-beta contains two polypeptides linked linked by disulfide bonds forming a protein of about 25 kDa.

Protocol

N/A

MSDS
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