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Reactivity | Human Mouse |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
Calculated MW | 37kDa |
Observed MW | Refer to figures |
Immunogen | Recombinant protein of human WDR77 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50; |
Immunohistochemistry of paraffin-embedded human prostate using WDR77 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using WDR77 antibody at dilution of 1:200 (40x lens).
Immunofluorescence analysis of A549 cell using WDR77 antibody. Blue: DAPI for nuclear staining.
WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002
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