BLVRA Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 295 amino acids (3-296 a.a. and Methionine at N-terminus) and having a molecular mass of 33.3kDa (molecular weight on SDS-PAGE will shift up).The BLVRA is purified by proprietary chromatographic techniques.
Biliverdin reductase A (BLVRA) is a member of the gfo/idh/mocA family. BLVRA is an enzyme that converts biliverdin to bilirubin, converting a double-bond between the second and third pyrrole ring into a single-bond. BLVRA reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IX alpha, to bilirubin with the simultaneous oxidation of a NADH or NADPH cofactor (Bilirubin + NAD(P)+ = biliverdin + NAD(P)H ).BLVRA is a regulator for induction of activating transcription factor-2 and heme oxygenase-1. Furthermore, BLVRA enhances the role of HO-1 in cytoprotection and provides cytoprotection independent of heme degradation. In addition, Bilirubin while acting as a cytoprotective antioxidant is itself oxidized to biliverdin and subsequently recycled by biliverdin reductase back to bilirubin.
Biliverdin reductase A, BVR A, Biliverdin-IX alpha-reductase, BLVRA, BLVR, BVR, BVRA.
Sterile Filtered colorless solution.
The BLVRA solution contains 20mM Tris-HCl buffer (pH8.0) and 10% glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.