COAA E.Coli Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 340 amino acids (1-316) and having a molecular mass of 38.9kDa.COAA is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
COAA is a member of the prokaryotic pantothenate kinase family and it is the first enzyme in the Coenzyme A biosynthetic pathway. COAA phosphorylates pantothenate (vitamin B5) to form 4'-phosphopantothenate. The key factor controlling the intracellular CoA concentration is the regulation of COAA activity by feedback inhibition.
PanK, ts-9, rts.
Sterile Filtered colorless solution.
The COAA solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM NaCl, 2mM DTT and 10% glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.