DARS Human-Synthetase

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DARS Human

Qty


Total
$65
Catalog #
ENPS-598
Size
Description
DARS Recombinant produced in E. coli is a single polypeptide chain containing 521 amino acids (1-501) and having a molecular mass of 59.3kDa.DARS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionDARS uses a 2 step reaction to catalyze the specific attachment of an amino acid to its cognate tRNA: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
SynonymsAspartyl-tRNA synthetase, Cell proliferation-inducing gene 40 protein, AspRS, aspartate tRNA ligase 1 cytoplasmic, EC 6.1.1.12.
SourceE.coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationThe DARS solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 150mM Nacl, 1mM DTT and 40% glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MPSASASRKS
QEKPREIMDA
AEDYAKERYG
ISSMIQSQEK
PDRVLVRVRD
LTIQKADEVV
WVRARVHTSR
AKGKQCFLVL
RQQQFNVQAL
VAVGDHASKQ
MVKFAANINK
ESIVDVEGVV
RKVNQKIGSC
TQQDVELHVQ
KIYVISLAEP
RLPLQLDDAV
RPEAEGEEEG
RATVNQDTRL
DNRVIDLRTS
TSQAVFRLQS
GICHLFRETL
IN
PurityGreater than 85% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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