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+1-888.733.6849
+1-617.299.7367 (Int’l)
Introduction | NULL |
Synonyms | Thermostable dUTPase, dUTPase. |
Source | Escherichia Coli. |
Physical Appearance | Sterile filtered liquid formulation. |
Formulation | dUTPase is supplied in 20mM Tris-HC1 (pH 8.2), 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 and 50% glycerol at concentration of 10 u/ul of the enzyme. |
Stability | Two years when stored at -20°C, 2 weeks at 4°C. |
Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Biological Activity | (A) Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma.(B) Enhancing PCR amplication: 50ul of Pfu PCR reaction system with 1-3u of dUTPase (3.0kb) to amplify genomic DNA target up to 15-19 kb in length. (High concentrations of dUTPase will inhibit PCR reaction!) |
Usage | NeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals. |
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