EGF Human, Pichia-Epidermal Growth Factor

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EGF Human, Pichia

Qty


Total
$65
Catalog #
CYPS-339
Size
Description
Epidermal Growth Factor Human Recombinant produced in Pichia Pastoris is a single, glycosylated, polypeptide chain containing 51 amino acids and having a molecular mass of 6KDa.The EGF is purified by proprietary chromatographic techniques.
Additional Information
IntroductionEpidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
SynonymsUrogastrone, URG, EGF.
SourcePichia Pastoris.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationEach lyophilized mg contains 0.15M NaCl, 0.025M sodium bicarbonate, pH-7.5.
SolubilityIt is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Epidermal Growth Factor Recombinant althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid SequenceThe
sequence
of
the
first
five
N-terminal
amino
acids
was
determined
and
was
found
to
be
Asn-Ser-Asp-Ser-Glu,
which
agrees
with
the
sequence
of
native
EGF
human.N-terminal
methionine
has
been
completely
removed
enzymatically.
PurityGreater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Biological ActivityThe ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml corresponding to a specific activity of 10MUnits/mg.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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