FGF 2 Human (147 a.a.)-Fibroblast Growth Factor

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FGF 2 Human (147 a.a.)

Qty


Total
$65
Catalog #
CYPS-564
Size
Description
Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16539 Dalton.The FGF2 is purified by proprietary chromatographic techniques.
Additional Information
IntroductionFGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thoµght to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
SynonymsProstatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationThe bFGF was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM Na2PO4, pH=8.
SolubilityIt is recommended to reconstitute the lyophilized FGF-B in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized basic-FGF althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGFb should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid SequenceMPALPEDGGS
GAFPPGHFKD
PKRLYCKNGG
FFLRIHPDGR
VDGVREKSDP
HIKLQLQAEE
RGVVSIKGVC
ANRYLAMKED
GRLLASKCVT
DECFFFERLE
SNNYNTYRSR
KYTSWYVALK
RTGQYKLGSK
TGPGQKAILF
LPMSAKS.
PurityGreater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Biological ActivityThe ED50, Calculated by the dose- dependent proliferation of mouse BALB/c 3T3 cells is 0.1-0.14ng/ml.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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