G CSF Human, CHO-Colony Stimulating Factor

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G CSF Human, CHO

Qty


Total
$65
Catalog #
CYPS-336
Size
Description
Granulocyte Colony Stimulating Factor Human Recombinant produced in CHO cells is a single, glycosylated, polypeptide chain containing 174 amino acids and having a molecular mass of approximately 18 kDa.G-CSF is purified by proprietary chromatographic techniques.
Additional Information
IntroductionGranulocyte Colony Stimulating Factor is a growth factor and/or cytokine produced by the endothelium, macrophages and a number of other immune cells. GCSF stimulates the bone marrow to produce granulocytes and also to stimulate the survival, proliferation, differentiation and function of neutrophil granulocyte progenator cells and mature neutrophils.
SynonymsCSF-3, MGI-1G, GM-CSF beta, Pluripoietin, Filgrastim, Lenograstim, G-CSF, MGC45931, GCSF.
SourceChinese Hamster Ovary Cells (CHO).
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationG-CSF was lyophilized from a concentrated (1mg/ml) Phosphate- Buffered Saline, pH 7.4.
SolubilityIt is recommended to reconstitute the lyophilized Granulocyte Colony Stimulating Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Granulocyte Colony Stimulating Factor althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution G-CSF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid SequenceTPLGPASSLP
QSFLLKCLEQ
VRKIQGDGAA
LQEKLCATYK
LCHPEELVLL
GHSLGIPWAP
LSSCPSQALQ
LAGCLSQLHS
GLFLYQGLLQ
ALEGISPELG
PTLDTLQLDV
ADFATTIWQQ
MEELGMAPAL
QPTQGAMPAF
ASAFQRRAGG
VLVASHLQSF
LEVSYRVLRH
LAQP.
PurityGreater than 97.0% as determined by:a) Analysis by RP-HPLC.b) Analysis by SDS-PAGE.
Biological ActivityThe ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.07 ng/ml, corresponding to a Specific Activity of 1.27 x 108 IU/mg.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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