G6PD E.Coli-Dehydrogenase

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G6PD E.Coli

Qty


Total
$65
Catalog #
ENPS-406
Size
Description
G6PD E.Coli Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 491 amino acids and having a molecular mass of 55.7kDa. The G6PD is purified by proprietary chromatographic techniques.
Additional Information
IntroductionG6PD is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-?-lactone and at the same time produces NADPH. The NADPH maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency causes acute hemolytic anemia, neonatal jaundice or acute hemolysis. G6PD is a cytosolic enzyme encoded by an X-linked gene whose main function is to produce NADPH, a crucial electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD produces pentose sµgars for nucleic acid synthesis and is a main producer of NADPH reducing power.
SynonymsG6PD, G6PD1, Glucose-6-phosphate 1-dehydrogenase.
SourceEscherichia Coli.
Physical AppearanceSterile filtered colorless solution.
FormulationThe G6PD protein contains 50mM MES 6.0, 0.1mM PMSF, 2mM EDTA, 0.5mM DTT and 10% glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMAVTQTAQAC
DLVIFGAKGD
LARRKLLPSL
YQLEKAGQLN
PDTRIIGVGR
ADWDKAAYTK
VVREALETFM
KETIDEGLWD
TLSARLDFCN
LDVNDTAAFS
RLGAMLDQKN
RITINYFAMP
PSTFGAICKG
LGEAKLNAKP
ARVVMEKPLG
TSLATSQEIN
DQVGEYFEEC
QVYRIDHYLG
KETVLNLLAL
RFANSLFVNN
WDNRTIDHVE
ITVAEEVGIE
GRWGYFDKAG
QM
PurityGreater than 90.0% as determined by SDS-PAGE.
Biological ActivitySpecific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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