GH Human, Plant-Growth Hormone

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GH Human, Plant

Qty


Total
$65
Catalog #
CYPS-060
Size
Description
GH human Recombinant produced in Nicotiana benthamiana plant is a single chain containing 205 amino acids (molecular formula C1025H1570N280O306S7) and 6-His-tag at the N-terminal having the total molecular mass of 22.9kDa.
Additional Information
IntroductionGH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thoµght to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.
SynonymsGH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin.
SourceNicotiana benthamiana plant
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationLyophilized from 1mg/ml solution in PBS 0.05M buffer at pH 7.5.
SolubilityIt is recommended to reconstitute the lyophilized GH in sterile 18M-cm H2O not less than 100
StabilityLyophilized GH althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Amino Acid SequenceHHHHHHFPTI
PLSRPFDNAM
LRAHRLHQLA
FDTYQEFEEA
YIPKEQKYSF
LQNPQTSLCF
SESIPTPSNR
EETQQKSNLE
LLRISLLLIQ
SWLEPVQFLR
SVFANSLVYG
ASDSNVYDLL
KDLEEGIQTL
MGRLEDGSPR
TGQIFKQTYS
KFDTNSHNDD
ALLKNYGLLY
CFRKDMDKVE
TFLRIVQCRS
VEGSCGFAG
PurityGreater than 97.0% as determined by Analysis by SDS-PAGE.
Biological ActivityThe biological activity of human Growth Hormone is measured by cell proliferation using Nb2-11 cells. ED50 < 0.04-0.1 ng/mL.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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