hchA E.Coli-Heat Shock Protein

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hchA E.Coli

Qty


Total
$65
Catalog #
HYPS-050
Size
Description
hchA produced in E.Coli is a single, non-glycosylated polypeptide chain containing 303 amino acids (1-283 a.a.) and having a molecular mass of 33.3kDa.hchA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionEscherichia coli Hsp31 (HchA) is a homodimeric member of the ThiI/DJ-1/PfpI superfamily which combines molecular chaperone and aminopeptidase activities. HchA uses temperature-induced exposure of structured hydrophobic domains to capture and stabilize early unfolding protein intermediates under severe thermal stress.
SynonymsChaperone protein hchA, EcHsp31, Hsp31, hchA, yedU, yzzC, b1967, JW1950.
SourceEscherichia Coli.
Physical AppearanceSterile filtered colorless solution.
FormulationThe hchA contains (1mg/ml) 20mM Tris-HCl buffer (pH8.0), 20% glycerol 0.1M NaCl and 1mM DTT.
StabilityhchA E.Coli Recombinant althoµgh stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MTVQTSKNPQ
VDIAEDNAFF
PSEYSLSQYT
SPVSDLDGVD
YPKPYRGKHK
ILVIAADERY
LPTDNGKLFS
TGNHPIETLL
PLYHLHAAGF
EFEVATISGL
MTKFEYWAMP
HKDEKVMPFF
EQHKSLFRNP
KKLADVVASL
NADSEYAAIF
VPGGHGALIG
LPESQDVAAA
LQWAIKNDRF
VISLCHGPAA
FLALRHGDNP
LNGYSICAFP
DA
PurityGreater than 95.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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