HSP27 Human, His-Heat Shock Protein

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HSP27 Human, His

Qty


Total
$65
Catalog #
HYPS-036
Size
Description
Recombinant Human HSP-27 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 225 amino acids (1-205 a.a.) and having a molecular mass of 24.9kDa. Hsp27 is fused to a 20 amino acid his tag at N-terminus and purified by conventional chromatography.
Additional Information
IntroductionHSP27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drµgs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38).
SynonymsHSPB1, CMT2F, HMN2B, HSP27, HSP28, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, Stress-responsive protein 27, SRP27, HS.76067, DKFZp586P1322.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationThe HSP27 protein solution contains 20mM Tris-Hcl buffer, 1mM DTT and 10% glycerol.
StabilityHSP-27 his tag althoµgh stable at 4°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MTERRVPFSL
LRGPSWDPFR
DWYPHSRLFD
QAFGLPRLPE
EWSQWLGGSS
WPGYVRPLPP
AAIESPAVAA
PAYSRALSRQ
LSSGVSEIRH
TADRWRVSLD
VNHFAPDELT
VKTKDGVVEI
TGKHEERQDE
HGYISRCFTR
KYTLPPGVDP
TQVSSSLSPE
GTLTVEAPMP
KLATQSNEIT
IPVTFESRAQ
LGGPEAAKSD
ETAAK.
PurityGreater than 95.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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