Interleukin-10 Human Recombinant produced in HEK cells is a glycosylated non-disulfide bonded homodimer, having a total molecular weight of 17kDa.The IL10 is purified by proprietary chromatographic techniques.
IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice sµggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
Sterile Filtered White lyophilized (freeze-dried) powder.
The IL10 was lyophilized from 1mg/ml in 10mM Tris-HCl, pH 7.4 and 150mM NaCl.
It is recommended to reconstitute the lyophilized IL-10 in sterile water not less than 100
Lyophilized IL-10 althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL10 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Greater than 95% as obsereved by SDS-PAGE.
The specific activity was determined by the dose-dependent stimulation of the proliferation of MC/9 cells (mouse mast cell line) in the presence of 200 pg/ml IL-4, and is typically 0.3-1.5ng/ml.
NeoScientifics products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.