KLK11 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain having a molecular weight of25.6kDa thoµgh on SDS-PAGE migrates at about 41kDa due to the glycosilation.The KLK11 is purified by proprietary chromatographic techniques.
KLK11 is a multifunctional protease. KLK11 cleaves ''bz-Phe-Arg-4-methylcoumaryl-7-amide'', KLK11 is a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. KLK11 cleaves synthetic peptides after arginine rather than lysine residues.
Sterile Filtered White lyophilized (freeze-dried) powder.
The KLK11 protein was lyophilized from a 0.2
It is recommended to reconstitute the lyophilized KLK11 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized KLK11 althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution KLK11 should be storedat 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
KLK11 specific activity is greater than 2200 pmoles/min/μg when measure with 100uM colormetric peptide substrate (D-Val-Leu-Lys-ThioBenzyl ester) and 1 μg of activated enzyme. The reaction is carried out in a volume of 100μL containing 50mM Tris, 1M NaCl, 10mM EDTA, 0.1mM DTNB (5,5’Dithio-bis(2-nitrobenzoic acid)), pH8.5 at 37°C. Cleavage of the peptide substrate can be measured at a wavelength of 405nm and quantified using the extinction coeffiecient 13,260M-1cm-1.
NeoScientific's products are furnished forLABORATORY RESEARCHUSEONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.