Beta-Lactamase TEM precursor Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 263 amino acids and having a molecular mass of 29 kDa. Beta Lactamase is purified by proprietary chromatographic techniques.
Beta-lactamase is a type of enzyme (EC 220.127.116.11) produced by some bacteria that is responsible for their resistance to beta-lactam antibiotics like penicillins, cephalosporins, cephamycins and carbapenems. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule''s antibacterial properties.
b-Lactamase, EC 18.104.22.168, TEM precursor.
Sterile Filtered White lyophilized (freeze-dried) powder.
Lyophilized from a concentrated (1 mg/ml) solution in water containing 20mM Phosphate buffer pH-7.
It is recommended to reconstitute the lyophilized Beta Lactamase in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Beta Lactamase althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Beta Lactamase Recombinant should be storedat 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.