Leptin tA Ovine-Leptin

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Leptin tA Ovine

Qty


Total
$65
Catalog #
CYPS-363
Size
Description
Leptin Antagonist Triple Mutant Ovine Recombinant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, Leptin was mutated, resulting in L39A/D40A/F41A mutant. Leptin Antagonist Triple Mutant Ovine Recombinant was purified by proprietary chromatographic techniques.
Additional Information
IntroductionNULL
SynonymsNULL
SourceEscherichia coli.
Physical AppearanceWhite lyophilized (freeze-dried) powder.
FormulationThe protein was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3.
SolubilityIt is recommended to reconstitute the lyophilized Leptin-Antagonist Triple Mutant Ovine Recombinant in sterile water or sterile 0.4% NaHCO3adjusted to pH 8, not less than 100
StabilityLyophilized Leptin Antagonist Triple Mutant Ovine Recombinant althoµgh stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 Lep-tA mutant mg/ml and up to 2 mM and filter sterilization Leptin mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is sµggested. Please prevent freeze-thaw cycles.
Amino Acid SequenceThe
sequence
of
the
first
five
N-terminal
amino
acids
was
determined
and
was
found
to
be
Ala-Val-Pro-Ile-Arg.
PurityGreater than 98.0% as determined by:(a) Gel filtration analysis.(b) Analysis by SDS-PAGE.
Biological ActivityNeoBiolab’s Leptin-Antagonist Triple Mutant Ovine Recombinant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of mouse leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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