LPL Human, HEK-Lipase

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LPL Human, HEK

Qty


Total
$65
Catalog #
ENPS-094
Size
Description
The Recombinant Human LPL produced in HEK293 cell line has a molecular mass of 51.8kDa containing 461 amino acid residues of the human LPL (Ala28-Gly475, variant Asn > Ser318) and fused to a 13 a.a. Flag-tag at N-terminus.
Additional Information
IntroductionLPL is a lipoprotein lipase, which is expressed in the heart, muscle, and adipose tissue. LPL acts as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Type I hyperlipoproteinemia is a result of severe mutations which cause LPL deficiency, whereas less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. Lipoprotein lipase (LPL) is a fundamental enzyme in plasma triglyceride hydrolysis and is secreted by macrophages in the subendothelial space. LPL also promotes the development of atherosclerosis throµgh facilitation of monocyte adhesion to endothelial cells, stimulation of tumor necrosis factor alpha (TNF) secretion and induction of vascular smooth muscle cell proliferation.
SynonymsLipoprotein lipase, LPL, LIPD, HDLCQ11.
SourceHEK293 (Human Embryonic Kidney cell line).
FormulationLPL was filtered (0.4
SolubilityIt is recomended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
StabilityStore lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Amino Acid SequenceHVDYKDDDDK
PAGADQRRDF
IDIESKFALR
TPEDTAEDTC
HLIPGVAESV
ATCHFNHSSK
TFMVIHGWTV
TGMYESWVPK
ADQRRDF
IDIESKFALR
TPEDTAEDTC
HLIPGVAESV
ATCHFNHSSK
TFMVIHGWTV
TGMYESWVPK
LVAALYKREP
DSNVIVVDWL
SRAQEHYPVS
AGYTKLVGQD
VARFINWMEE
EFNYPLDNVH
LLGYSLGAHA
AGIAGSLTNK
KVNRI
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
ApplicationsWestern blotting.
Background

N/A

Protocol

N/A

MSDS
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