Luciferase Firefly-Oxygenase

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Luciferase Firefly

Qty


Total
$65
Catalog #
ENPS-560
Size
Description
Luciferase produced in E.Coli is a single, non-glycosylated polypeptide chain containing 571 amino acids (1-550 a.a.) and having a molecular mass of 62.9kDa.Luciferase is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionLuciferase is a general term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. Luciferase catalyzes a bioluminescent reaction which involves the substrate luciferin as well as Mg2+ and ATP, produces green light with a wavelength of 562 nm. Luciferase from firefly is broadly used as a reporter for studying gene regulation and function, and for pharmaceutical screening.
SynonymsLuciferase-like monooxygenase, LUC, EC 1.13.12.7.
SourceEscherichia Coli.
Physical AppearanceSterile filtered colorless solution.
FormulationThe Luciferase protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH-8), 1mM DTT, and 10% glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MMEDAKNIKK
GPAPFYPLED
GTAGEQLHKA
MKRYALVPGT
IAFTDAHIEV
DITYAEYFEM
SVRLAEAMKRYGLNTNHRIV
VCSENSLQFF
MPVLGALFIG
VAVAPANDIY
NERELLNSMG
ISQPTVVFVS
KKGLQKILNV
QKKLPIIQKI
IIMDSKTDYQGFQSMYTFVT
SHLPPGFNEY
DFVPESFDRD
KTIALIMNSS
GSTGLPKGVA
LPHR
PurityGreater than 90.0% as determined by SDS-PAGE.
UsageNeoScientifics products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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