MAPKAPK3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 405 amino acids (1-382 a.a) and having a molecular mass of 45.4kDa. MAPKAPK3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
MAP kinase-activated protein kinase 3 (MAPKAPK3) is involved in inflammatory Reaction by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally. MAPKAPK3 phosphorylates AU-rich elements (AREs)-binding proteins, like TTP/ZFP36, leading to control of stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36 (a major post-transcriptional regulator of TNF), promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity resulting in inhibition of dependent degradation of ARE-containing transcript. MAPKAPK3 is activated by growth inducers and stress stimulation of cells.
3PK, MAPKAP-K3, MAPKAP3, MAPKAPK-3, MK-3, MAP kinase-activated protein kinase 3, MAPK-activated protein kinase 3, MAPKAP kinase 3,MAPKAPK-3, MK-3,MAPKAPK3.
Sterile Filtered colorless solution.
MAPKAPK3 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 20% glycerol and 1mM DTT.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.