NEDD8 Human-Ubiquitin Conjugating Enzyme

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NEDD8 Human

Qty


Total
$65
Catalog #
ENPS-403
Size
Description
NEDD8 Human Recombinant fused with 37 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 113 amino acids (1-76 a.a.) and having a molecular mass of 12.8 kDa.The NEDD8 is purified by proprietary chromatographic techniques.
Additional Information
IntroductionNEDD8 is part of the ubiquitin family. Human NEDD8 shares 60% amino acid sequence homology to ubiquitin. The NEDD8 system is essential for the regulation of protein degradation pathways involved in cell cycle progression, morphogenesis and tumorigenesis. NEDD8 is involved in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E-1 complex UbE1c- appbp1 and linkage to the E-2 enzyme UbE2m. Attachment of NEDD8 to cullins activates their associated E-3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
SynonymsNedd-8, FLJ43224, MGC104393, MGC125896, MGC125897, NEDD8, Ubiquitin-like protein Nedd8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationThe NEDD8 solution contains 20mM Tris pH 8.0, 50mM NaCl, 0.5mM DTT & 10% glycerol.
StabilityNEDD8 althoµgh stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid SequenceMHHHHHHMKI
EEGKLVIWIN
GDKGYNGLAE
VGKKFEKDTG
IKVTVEHPDK
LEEKFPQVAA
TGDGPDIIFW
AHDRFGGYAQ
SGLLAEITPD
KAFQDKLYPF
TWDAVRYNGK
LIAYPIAVEA
LSLIYNKDLL
PNPPKTWEEI
PALDKELKAK
GKSALMFNLQ
EPYFTWPLIA
ADGGYAFKYE
NGKYDIKDVG
VDNAGAKAGL
TFLVDLIKNK
HMNADTDYSI
AEAAFNKGET
AM
PurityGreater than 95.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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