NFNB Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 237 amino acids (1-217 a.a.) and having a molecular mass of26 kDa. The NFNB is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
NFNB demonstrates the capability to reduce quinines. NFNB enzyme activates prodrµgs in antibody directed enzyme prodrµg therapy. NFNB reduces nitrofurazone, quinones and anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species.