NIT2 Human-Other

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NIT2 Human

Qty


Total
$65
Catalog #
ENPS-046
Size
Description
NIT2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 299 amino acids (1-276a.a.) and having a molecular mass of 33kDa.NIT2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionNIT2 is a member of the nitrilase superfamily. NIT2 protein has an omega-amidase activity which removes potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. In addition, Nit2 is widely distributed in nature and is thoµght to be a tumor suppressor protein.
SynonymsNitrilase homolog 2, Nitrilase family member 2, omega-amidase NIT2, MGC111199, Nit protein 2, EC 3.5.1.3.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered clear solution.
FormulationThe NIT2 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0) 1mM DTT, 0.1M NaCl, and 10% glycerol.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MGSMTSFRLA
LIQLQISSIK
SDNVTRACSF
IREAATQGAK
IVSLPECFNS
PYGAKYFPEY
AEKIPGESTQ
KLSEVAKECS
IYLIGGSIPE
EDAGKLYNTC
AVFGPDGTLL
AKYRKIHLFD
IDVPGKITFQ
ESKTLSPGDS
FSTFDTPYCR
VGLGICYDMR
FAELAQIYAQ
RGCQLLVYPG
AFNLTTGPAH
WELLQRSRAV
DNQVYVATAS
PA
PurityGreater than 95% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
StorageStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.
Background

N/A

Protocol

N/A

MSDS
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