PSMB3 Human-Proteasome

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PSMB3 Human

Qty


Total
$65
Catalog #
ENPS-207
Size
Description
PSMB3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 225 amino acids (1-205 a.a.) and having a molecular mass of 25.1kDa.PSMB3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionThe proteasome is a multicatalytic proteinase complex with an extremely organized ring-shaped 20S core structure. The core structure consists of 4 rings of 28 non-identical subunits; 2 rings consist of 7 alpha subunits and 2 rings consist of 7 beta subunits. Proteasomes are spread all over the eukaryotic cells in large quantities and cleave peptides in an ATP/ubiquitin-dependent procedure in a non-lysosomal pathway. A vital role of a modified proteasome, the immunoproteasome, is the handling of class I MHC peptides.
SynonymsProteasome (prosome, macropain) subunit beta type 3, Proteasome chain 13, HC10-II, Proteasome component C10-II, Proteasome theta chain, EC 3.4.25.1, MGC4147.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationPSMB3 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 200mM NaCl, 1mM DTT and 50% glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MSIMSYNGGA
VMAMKGKNCV
AIAADRRFGI
QAQMVTTDFQ
KIFPMGDRLY
IGLAGLATDV
QTVAQRLKFR
LNLYELKEGR
QIKPYTLMSM
VANLLYEKRF
GPYYTEPVIA
GLDPKTFKPF
ICSLDLIGCP
MVTDDFVVSG
TCAEQMYGMC
ESLWEPNMDP
DHLFETISQA
MLNAVDRDAV
SGMGVIVHII
EKDKITTRTL
KARMD
PurityGreater than 90.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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