PYCRL Human-Reductase

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PYCRL Human

Qty


Total
$65
Catalog #
ENPS-685
Size
Description
PYCRL Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 297 amino acids (1-274) and having a molecular mass of 31kDa.PYCRL is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionPyrroline-5-Carboxylate Reductase Like (PYCRL) is a member of the pyrroline-5-carboxylate reductase family and acts as a homodecamer. PYCRL plays a key role in proline bio-synthesis. Proline serves as a non-enzymatic antioxidant to reduce damage caused by reactive oxygen species (ROS) in microorganisms, animals and plants. In the final stage of proline biosynthesis, PYCRL catalyzes the reduction of aldehyde dehydrogenase 4A1 (ALDH4A1) to proline with NAD(P)H as the cofactor.
SynonymsPyrroline-5-carboxylate reductase 3, P5C reductase 3, P5CR 3, Pyrroline-5-carboxylate reductase-like protein, PYCRL.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationThe PYCRL solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol and 2mM DTT.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MGSMAAAEPS
PRRVGFVGAG
RMAGAIAQGL
IRAGKVEAQH
ILASAPTDRN
LCHFQALGCR
TTHSNQEVLQ
SCLLVIFATK
PHVLPAVLAE
VAPVVTTEHI
LVSVAAGVSL
STLEELLPPN
TRVLRVLPNL
PCVVQEGAIV
MARGRHVGSS
ETNLLQHLLE
ACGRCEEVPE
AYVDIHTGLS
GSGVAFVCAF
SEALAEGAVK
MGMPSSLAHR
IA
PurityGreater than 90.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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