pykF E.Coli-Protein Kinases

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pykF E.Coli

Qty


Total
$65
Catalog #
PKPS-035
Size
Description
pykF E.Coli Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 494 amino acids (1-470 a.a) and having a molecular mass of 53.3kDa.pykF is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionpykF (pyruvate kinase I) is a member of the pyruvate kinase family. pykF is an enzyme involved in glycolysis. pykF catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP, a process which also requires a Magnesium ion. This is the final step in the glycolytic pathway, which produces pyruvate molecules, the ultimate product of aerobic glycolysis.
SynonymsPyruvate kinase I, PK-1, pykF, b1676, JW1666.
SourceE.coli.
Physical AppearanceSterile Filtered colorless solution.
FormulationpykF protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0) and 10% glycerol.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MGSHMKKTKI
VCTIGPKTES
EEMLAKMLDA
GMNVMRLNFS
HGDYAEHGQR
IQNLRNVMSK
TGKTAAILLD
TKGPEIRTMK
LEGGNDVSLK
AGQTFTFTTD
KSVIGNSEMV
AVTYEGFTTD
LSVGNTVLVD
DGLIGMEVTA
IEGNKVICKV
LNNGDLGENK
GVNLPGVSIA
LPALAEKDKQ
DLIFGCEQGV
DFVAASFIRK
RSDVIEIREH
LK
PurityGreater than 95% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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