SCF Human-Stem Cell Factor

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SCF Human

Qty


Total
$65
Catalog #
CYPS-262
Size
Description
Stem Cell Factor Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton. The SCF is purified by proprietary chromatographic techniques.
Additional Information
IntroductionStem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases. SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).
SynonymsKit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationLyophilized from a concentrated (1mg/ml) solution in water containing 0.02% NaHCO3.
SolubilityIt is recommended to reconstitute the lyophilized Stem Cell Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized KIT ligand althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid SequenceThe
sequence
of
the
first
five
N-terminal
amino
acids
was
determined
and
was
found
to
be
Met-Glu-Gly-Ile-Cys.
PurityGreater than 98.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE.
Biological ActivityThe ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is < 2 ng/ml, corresponding to a Specific Activity of 500,000 IU/mg.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
References1.Title:Direct binding of Cbl to Y568 and Y936 of the stem cell factor receptor/c-Kit is required for ligand-induced ubiquitination, internalization and degradation.Publication:Biochemical Journal Immediate Publication. Published on 19 Jun 2006 as manuscript BJ20060464.Link:http://www.biochemj.org/bj/imps_x/pdf/BJ20060464.pdf2.Title:The D816V Mutation of c-Kit Circumvents a Requirement for Src Family Kinases in c-Kit Signal Transduction.Publication: March 5, 2009, doi: 10.1074/jbc.M808058200 April 24, 2009 The Journal of Biological Chemistry, 284, 11039-11047. Link:http://www.jbc.org/content/284/17/11039.full3.Title:Structural Basis for c-KIT Inhibition by the Suppressor of Cytokine Signaling 6 (SOCS6) Ubiquitin Ligase.Publication:First Published on October 28, 2010, doi: 10.1074/jbc.M110.173526 January 7, 2011 The Journal of Biological Chemistry, 286, 480-490. Link:http://www.jbc.org/content/286/1/480.full
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