TIGAR Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 283 amino acids (including the 270 residues of full-length TIGAR and a 13-residue C-terminal TAT peptide) and having a molecular mass of 31.7kDa. The TIGAR is purified by proprietary chromatographic techniques.
TIGAR is a p53-inducible enzyme which catalyzes the hydrolysis of fructose-2-6 bisphosphate (F-2-6-BP) to fructose-6-phosphate and inorganic phosphate. F-2-6-BP is an influential activator of 6-phosphofructose-1 kinase (the rate limiting enzyme of glycolysis). By lowering the intracellular level of F-2-6-BP, TIGAR expression leads to increased glucose processing throµgh the pentose phosphate pathway, the main cellular source for NADPH. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other intracellular proteins into primary as well as transformed cells.
Fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, TIGAR, C12orf5.
Sterile Filtered White lyophilized (freeze-dried) powder.
TIGAR was Lyophilized from a 0.2
It is recommended to reconstitute the lyophilized TIGAR in sterile 18M-cm H2O not less than 100
Lyophilized TIGAR stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TIGAR should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.