TIGAR Human, TAT-Other

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TIGAR Human, TAT

Qty


Total
$65
Catalog #
PRPS-1587
Size
Description
TIGAR Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 283 amino acids (including the 270 residues of full-length TIGAR and a 13-residue C-terminal TAT peptide) and having a molecular mass of 31.7kDa. The TIGAR is purified by proprietary chromatographic techniques.
Additional Information
IntroductionTIGAR is a p53-inducible enzyme which catalyzes the hydrolysis of fructose-2-6 bisphosphate (F-2-6-BP) to fructose-6-phosphate and inorganic phosphate. F-2-6-BP is an influential activator of 6-phosphofructose-1 kinase (the rate limiting enzyme of glycolysis). By lowering the intracellular level of F-2-6-BP, TIGAR expression leads to increased glucose processing throµgh the pentose phosphate pathway, the main cellular source for NADPH. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other intracellular proteins into primary as well as transformed cells.
SynonymsFructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, TIGAR, C12orf5.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationTIGAR was Lyophilized from a 0.2
SolubilityIt is recommended to reconstitute the lyophilized TIGAR in sterile 18M-cm H2O not less than 100
StabilityLyophilized TIGAR stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TIGAR should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Amino Acid SequenceMARFALTVVR
HGETRFNKEK
IIQGQGVDEP
LSETGFKQAA
AAGIFLNNVK
FTHAFSSDLM
RTKQTMHGIL
ERSKFCKDMT
VKYDSRLRER
KYGVVEGKAL
SELRAMAKAA
REECPVFTPP
GGETLDQVKM
RGIDFFEFLC
QLILKEADQK
EQFSQGSPSN
CLETSLAEIF
PLGKNHSSKV
NSDSGIPGLA
ASVLVVSHGA
YMRSLFDYFL
TDLKCSLPAT
LSRSELMSVT
PN
PurityGreater than 95.0% as determined by: (a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Biological ActivityThe Specific Activity was measured by its ability to protect U2OS cells from apoptosis induced by hydrogen peroxide is in a concentration range of 0.1-5.0
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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