TPK1 Human-Protein Kinases

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TPK1 Human

Qty


Total
$65
Catalog #
PKPS-030
Size
Description
TPK1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 267 amino acids (1-243 a.a.) and having a molecular mass of 29.8kDa.TPK1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionThiamin pyrophosphokinase 1 (TPK1) is a homodimer which catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. TPK1 is found in the heart, kidney, testis, small intestine and peripheral blood leukocytes, and at very low levels in a variety of tissues. TPK1 gene defects cause the thiamine metabolism dysfunction syndrome type 5, episodic encephalopathy type (THMD5), which is an autosomal recessive metabolic disorder due to an inborn error of thiamine metabolism.
SynonymsThiamin pyrophosphokinase 1, hTPK1, Placental protein 20, PP20, Thiamine pyrophosphokinase 1, TPK1, THMD5.
SourceEscherichia Coli.
Physical AppearanceSterile filtered colorless solution.
FormulationTPK1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 1mM DTT.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MGSHMEHAFT
PLEPLLSTGN
LKYCLVILNQ
PLDNYFRHLW
NKALLRACAD
GGANRLYDIT
EGERESFLPE
FINGDFDSIR
PEVREYYATK
GCELISTPDQ
DHTDFTKCLK
MLQKKIEEKD
LKVDVIVTLG
GLAGRFDQIM
ASVNTLFQAT
HITPFPIIIIQEESLIYLLQ
PGKHRLHVDT
GMEGDWCGLI
PVGQPCMQVT
TTGLKWNLTN
DVL
PurityGreater than 90.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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