UBE2H Human-Ubiquitin Conjugating Enzyme

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UBE2H Human

Qty


Total
$65
Catalog #
ENPS-610
Size
Description
UBE2H Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 206 amino acids (1-183) and having a molecular mass of 23.1kDa.UBE2H is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Additional Information
IntroductionUbiquitin-conjµgating enzyme E2 H (UBE2H) is a member of the ubiquitin-conjµgating enzyme family. Protein modification with ubiquitin is a vital cellular apparatus for directing abnormal or short-lived proteins for degradation. Ubiquitination requires at least 3 classes of enzymes: ubiquitin-activating enzymes (E1s) ubiquitin-conjµgating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2H receives ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. UBE2H protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs.
SynonymsUbiquitin-conjµgating enzyme E2 H, UbcH2, Ubiquitin carrier protein H, Ubiquitin-conjµgating enzyme E2-20K, Ubiquitin-protein ligase H, UBE2H, GID3, UBC8, UBCH.
SourceEscherichia Coli.
Physical AppearanceSterile filtered colorless solution.
FormulationThe UBE2H solution (1mg/ml) contains 20mM Tris-HCl buffer, pH8.0, 10% glycerol, 1mM DTT and 50mM NaCl.
StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino Acid SequenceMGSSHHHHHH
SSGLVPRGSH
MGSMSSPSPG
KRRMDTDVVK
LIESKHEVTI
LGGLNEFVVK
FYGPQGTPYE
GGVWKVRVDL
PDKYPFKSPS
IGFMNKIFHP
NIDEASGTVC
LDVINQTWTA
LYDLTNIFES
FLPQLLAYPN
PIDPLNGDAA
AMYLHRPEEY
KQKIKEYIQK
YATEEALKEQ
EEGTGDSSSE
SSMSDFSEDE
AQDMEL.
PurityGreater than 95.0% as determined by SDS-PAGE.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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