VEGF Human, HEK-VEGF

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VEGF Human, HEK

Qty


Total
$65
Catalog #
CYPS-232
Size
Description
Vascular Endothelial Growth Factor Human Recombinant produced in HEK293 cells is a double, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 40 kDa.The VEGF is purified by proprietary chromatographic techniques.
Additional Information
IntroductionVascular endothelial growth factor is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, althoµgh it does have effects on a number of other cell types (e.g. stimulation monocyte/ macrophagemigration, neurons, cancer cells, kidney epithelial cells ).VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesisand cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor.Elevated levels of this protein are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.
SynonymsVascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
SourceHEK293 (Human Embryonic Kidney cell line).
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationThe protein was lyophilized from a concentrated (1mg/ml) solution with no additives.
SolubilityIt is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor-HEK in sterile 18M-cm H2O not less than 100
StabilityLyophilized Vascular Endothelial Growth Factor HEK althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF HEK should be storedat 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid SequenceAPMAEGGGQN
HHEVVKFMDV
YQRSYCHPIE
TLVDIFQEYP
DEIEYIFKPS
CVPLMRCGGC
CNDEGLECVP
TEESNITMQI
MRIKPHQGQH
IGEMSFLQHN
KCECRPKKDR
ARQENPCGPC
SERRKHLFVQ
DPQTCKCSCK
NTDSRCKARQ
LELNERTCRC
DKPRR.
PurityGreater than 95.0% as determined by SDS-PAGE.
Biological ActivityDetermined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-6.0 ng/ml, corresponding to a specific activity of 1 x 106 Units/mg to 1.7 x 105 Units/mg.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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