HA-Tag-Monoclonal Antibodies

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HA-Tag

Qty


Total
$135
Catalog #
AE008
Antibody Type
Monoclonal Antibody
Gene ID
Swiss Prot
Size
Species
Mouse
Isotype
IgG
Purity
Affinity purification
Additional Information
Tested applicationsWB IP
Recommended DilutionWB 1:2000 - 1:5000 IP 1:20 - 1:50
Observed MWRefer to Figures
ImmunogenA synthetic peptide of YPYDVPDYA (HA-tag)-KLH
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Images
  • AE008: image 1

    Western blot analysis of over-expressed HA-tagged protein in 293T cell using HA antibody at different dilution. Each lane was loaded with 2 ug cell lysate.

Background

Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. For example, the pCDM8 expression vector and derivatives thereof encode fusions between the target protein and an 11 amino acid peptide derived from the influenza protein hemagglutinin (HA). The HA epitope tag is useful in Western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-epitope tag.

Protocol

N/A

MSDS
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