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Rabbit Monoclonal Antibodies

The Neo Rabbit-mAb™ developed 3.0 generation of recombinant Rabbit Monoclonal Antibody is exclusively available from NEO Scientific. Rabbit-mAb™ employs innovative cell sorting, enriching, bioinformatics, and recombinant monoclonal antibody technologies. NEO Scientific selects the desired antibody directly from B cells.

Our technologies provide more candidates that are unreachable by traditional technologies - our in-house bioinformatics optimizes the selection, our new generation of gene synthesis solutions cuts down the cost of antibody engineering, and our XPromoter 2.0 can highly express recombinant antibodies.

NEO provides antibodies with high specificity and sensitivity in multiple applications and manufactures cost-efficient large-scale antibodies.

CUSTOM SERVICES OFFER YOU:

  • High-quality custom antibodies tailored to your applications
  • Managed by PhD-level scientist
  • Opportunities to perform sample testing at various stages
  • Implemented in Cambridge, Massachusetts, not offshore

TO WORK WITH US, PLEASE PROVIDE:

  • The antigen information: NEO will produce the immunogens for you, such as peptides, recombinant proteins, etc
  • Prepared immunogens: such as peptides, proteins, small molecules, or other samples.
  • Spleen and/ or blood of an immunized rabbit: NEO will initiate the custom monoclonal antibody development.

We also provide comprehensive services for antibody cloning, engineering, expression, and purification in addition to custom antibody development.

Timeline

Phase I: Immunization

Phase II: Cell Sorting, Screening, Sequencing

Phase III: Cloning, Expression, and Characterization

Project Phases Time Frame
I. Immunization
  • Immunization of 2 Rabbits
  • Antigen requirements:
  • 4mg of protein antigen
  • 2mg KLH & 2mg BSA conjugated peptide
 		60-80 Days
II. Screening and Selection
  • Isolation and culture of B-cells
  • Identification of specific B lymphocytes by Indirect ELISA
  • Enrichment of target antibody-secreting cells with high binding affinity to antigen
  • Sequencing of rabbit IgG genes
 		30-40 Days
III. Cloning & Expression
  • Cloning of heavy and light chains into separate expression vectors
  • Transfection of heavy and light chain plasmids in mammalian cell line for antibody expression
  • Identification of antigen-specific antibodies by ELISA
 		25-35 Days
Deliverables:
2-3mg of four different recombinant rabbit monoclonal antibodies
(guarantee indirect ELISA)
  • Full-length antibody genes of selected
  • Clones in plasmid format
 		1-3 days
1 ADVANTAGES OF RABBIT MONOCLONAL ANTIBODIES

The rabbit immune system generates antibody diversity and optimizes affinity via different mechanisms than rodents. Neo Ra-mAb 3.0 develops antiobodies that not only recognize antigens efficiently, but also with specificity and consistency. On average, our antibodies have 10-100x higher affinity than mouse monoclonals. Immunization of rabbits results in a highly diverse suite of B-lymphocytes and an abundance of splenocytes. The breadth of available cells provides greater opportunities to screen antibodies that distinguish between similar molecules and improve specificity accordingly.

  • More diverse epitope recognition
  • Low background signals
  • Improved immune response to small epitopes
  • High specificity and affinity
  • Greatly improved response to mouse antigens
  • Able to differentiate between subtle structural variations
  • Ideal for post-translational modification detection
  • Fit for low-abundance biomarker quantification
  • Convenient for use with mouse proteins and tissues
2 THE NEO RA-MAB™ 3.0 ADVANTAGES
  • No the traditional cell fusion step
  • No stability issues associated with hybridomas
  • Recombinant antibody ensures constant production
  • Can provide both monoclonal and polyclonal antibodies
  • Flexible optimization at each stage
  • High and cost-efficient production

Quote

  • For quotation requests and questions, you may contact us by email, phone, or via our online quotation system.
  • Orders can be placed by phone, email, fax, or online with a formal Purchase Order or credit card.
  • Please send your samples to NeoScientific, 245 First Street, 18th Floor, Cambridge, MA 02142 USA.
  • Our customer service is available 24hours per day/7 days a week/365 days a year.

  • Our customer service is available 24/7/365.
  • You may contact us anytime for assistance:
  • Email: sales@neoscientific.com
  • +1-888.733.6849, +1-617.299.7367 (Int'l)