Custom Phospho Specific Antibody Production

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Quotations & Ordering:

For quotations, please use our online quotation form, and you may also contact us by

[email protected]

Phone:

+1888-754-5670(Toll-free)
+1857.259.4898 (Int’l)

Fax:

+1888-754-5670,

Our customer service representatives are available 24 hours, Monday through Friday to assist you.
Phospho-specific antibodies are widely used to identify the activation of signal transduction pathways resulting from the phosphorylation of a diverse set of proteins that include enzymes, receptors, and transcription factors. We have extensive experience developing high-specificity phospho-specific antibodies for our customers.
Polyclonal
Package includes:

Peptide synthesis: Phospho-amino acid modified peptide (>15 aa >90% purity, 15 mg) and unphosphorylated control (>15 aa >90% purity, 11 mg)
Animal immunization: 2 rabbits, multiple production bleeds
Phospho-specific ELISA
Affinity purification with phosphopeptide and cross-adsorption with non-phospho peptide
Lyophilization (optional)

You will receive (in 15-17 weeks):

1-30 mg purified antibody
5 mg phophopeptide
5 mg non-phospho peptide
1-2 ml of pre-immune serum
ELISA results, MS and HPLC reports

Monoclonal
Package includes:

Peptide synthesis: Phospho-amino acid modified peptide (>15 aa >90% purity, 10 mg) and unphosphorylated control (>15 aa >90% purity, 6 mg)
Animal immunization: 10 mice or 6 rats
Test bleed ELISA and hybridoma screening for phophopeptide, non-phosphopeptide and generic phosphopeptide
Subcloning, expansion, cryopreservation of positive cell lines
Lyophilization of supernatants

You will receive (5-7 months):

2 positive hybridoma clones
5 ml positive supernatant for each clone
5 mg phophopeptide
5 mg non-phospho peptide

NeoScientific's standard option for polyclonal antibody is optimized to produce antiserum in the shortest possible time. It is the most affordable option, popular among researchers.

The protocol uses two New Zealand White rabbits that are Specific Pathogen-Free. Injections are subcutaneous (SQ) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA).

Procedure Protocol Day Description
Control Serum Collection Day 0 Pre-immune bleed (5mL per rabbit)
Primary Injection Day 1 Immunize with 0.50mg of antigen in CFA, 10 SQ sites
1st Booster Day 14 Boost with 0.25mg of antigen in IFA, 4 SQ sites
2nd Booster Day 28 Boost with 0.25mg of antigen in IFA, 4 SQ sites
Serum Collection Day 35 Bleed (~25mL per rabbit)
3rd Booster Day 42 Boost with 0.25mg of antigen in IFA, 4 SQ sites
Serum Collection Day 56, 58 Two bleeds (~50mL total per rabbit)
ELISA Test Day 60 ELISA titration (results available online);
Verify disposition of rabbits; decide to continue or terminate
Purification Optional 6-10 mg purified antibodies
Quote
  • For quotation requests and questions, you may contact us by email, phone, or via our online quotation system.
  • Orders can be placed by phone, email, fax, or online with a formal Purchase Order or credit card.
  • Please send your samples to NeoScientific, 245 First Street, 18th Floor, Cambridge, MA 02142 USA.
  • Our customer service is available 24hours per day/7 days a week/365 days a year.

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