GM CSF Monkey-Colony Stimulating Factor

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GM CSF Monkey

Qty


Total
$65
Catalog #
CYPS-727
Size
Description
Granulocyte Macrophage Colony Stimulating Factor Monkey Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14.4 kDa.GM-CSF is purified by proprietary chromatographic techniques.
Additional Information
IntroductionGMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
SynonymsCSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim,MGC131935, MGC138897.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationGM-CSF was lyophilized after extensive dialysis against 1x PBS pH= 7.4
SolubilityIt is recommended to reconstitute the lyophilized GMCSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized GMCSF althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GMCSF should be storedat 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid SequenceAPARSPSPGT
QPWEHVNAIQ
EARRLLNLSR
DTAAEMNKTV
EVVSEMFDLQ
EPSCLQTRLE
LYKQGLQGSL
TKLKGPLTMM
ASHYKQHCPP
TPETSCATQI
ITFQSFKENL
KDFLLVIPFD
CWEPVQE.
PurityGreater than 95.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.
Biological ActivityThe ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.1 ng/ml, corresponding to a Specific Activity of 10,000,000 IU/mg.
UsageNeoScientific's products are furnished forLABORATORY RESEARCHUSEONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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